Objective To analyze the epidemiological characteristics and sources of infection of human brucellosis, as well as the incidence of brucellosis among high-risk occupational populations, in Miyun district of Beijing, China, from 2013 to 2015, and to provide a basis for the prevention and control of brucellosis. Methods All brucellosis cases reported in Miyun district of Beijing from 2013 to 2015 were investigated, and the high-risk occupational populations were analyzed by serological epidemiology. The chi-square test was used to compare the rates between groups. Results A total of 48 brucellosis cases were reported in Miyun district from 2013 to 2017. The mean annual incidence of brucellosis was 2.00/100 000, with the peak period from April to July. There were more males than females among the reported cases. The 40-59 years old group had higher number of cases and incidence of brucellosis than other age groups. Of all cases, 77.08% were farmers, and 81.25% were infected by sheep. The serum antibody positive rate was 2.72% in the high-risk occupational populations of brucellosis in Miyun district from 2013 to 2017. Conclusion The sheep was the main source of infection for human brucellosis cases reported in Miyun district from 2013 to 2017, and the infection rate of brucellosis was relatively high in the high-risk occupational populations.

Objective To investigate the infection status in arthritis patients with Lyme disease in Miyun district, and to provide scientific basis for the prevention and treatment of Lyme disease. Methods Serum samples of patients with arthritis were collected, Lyme disease antibody screening in serum samples were detected by enzyme-linked immunosorbent assay, and positive serum samples were identified by western blot. Results A total of 140 serum samples were collected, 17 samples were positive of Lyme disease antibody, the total positive rate was 12.14%. Four serum samples were positive for Lyme disease IgM antibody, the positive rate was 2.86%, 13 serum samples were positive for Lyme disease IgG antibody, the positive rate was 9.29%. The total positive rate of antibody was higher in women than men, and the total positive rate of antibody was highest in 50- years old age group, there were no significant differences in total positive rate between different districts. Conclusion There were patients with arthritis caused by the Borrelia burgdorferi infection in Miyun district, so clinicians should consider Lyme disease in the diagnosis and treatment of arthritis patients.

Objective To explore the infection status and the characteristics of distribution of Orientia tsutsugamushi in the north mountainous area of Beijing, and to provide a scientific basis for its prevention and treatment. Methods A total of 549 subjects were selected to carry out the seroepidemiological investigation from April to August of 2011, enzyme-linked immunosorbent assay test(ELISA) method was used to detect the scrub typhus IgG antibody. Meanwhile, in accordance with different geographical region, different age and different sex, the antibody level of the serum samples were made a comparison, and the survey questionnaire was carried out. Results A total of 549 serum samples were collected, the positive rate of scrub typhus IgG antibody was 9.47%. The positive rates of the male and female antibody were 11.06% (26/235)and 8.28%(26/314), respectively. The positive rate of scrub typhus IgG antibody in each monitoring point were Dachengzi 10.00%, Taishitun 9.33%, Xitiangezhuang 15.00%, Gubeikou town 5.00%, Shicheng town 8.08%, respectively. And there were no significant differences in antibody levels of human serum samples in different regions, age and sex groups(χ²=6.156, 6.307, 1.215, P=0.188, 0.177, 0.270). The survey found that only 8 persons knew of scrub typhus, 104 of them could recognize the chigger, and only 41 of them had an obvious chigger bite history. People knew scrub typhus through TV. Conclusion Orientia tsutsugamushi is in endemic in the north mountainous area of Beijing, and the distribution of the regions is wide. Natural foci of scrub typhus likely exist in the north mountainous area of Beijing; and the exposed populations lack of knowledge of scrub typhus and have high infection risk, so it is urgent to take the health education in rural area by various ways.

【Abstract】 Objective To understand the situation of the reservoir hosts and vectors infected by Borrelia burgdorferi, the distribution of the reservoir hosts and the seasonal fluctuation of ticks in Miyun area.  Methods Ticks were caught with flag method, which were identified by Inverted microscope. The night trapping method was used to catch rats.  The pathogen from ticks and rodents was isolated with BSKⅡculture. The nested PCR was used to do etiological detection. The antibody of B.burgdorferi from goat serum was tested by indirect immunofluorescence. Results Ticks caught in Miyun area were all Haemaphysalis longicornis, which growth curve was obviously seasonal. The rate of ticks bearing pathogen was 10.16% after detection by nested PCR. Apodemus agrarius and Niniventer confucianus were the dominant species in the area, and the rate of rodents bearing pathogen was  4.26%.  The  positive  rate  of  goats  stocked  in  the  local  was  12.00%.  Conclusion The  specific fragment of B.burgdorferi was found in ticks and rodents, which showed that Miyun area maybe one of the epidemic focus of Lyme disease.